Prepare a heavy suspension of a pure culture in 2 ml of 0.85% saline.Procedure of Bile Solubility Test Tube Method Dissolve 10 gram of sodium deoxycholate into 100 ml sterile distilled water.Dissolve 2 gram of sodium deoxycholate into 100 ml sterile distilled water.ġ0% sodium deoxycholate (bile salt) solution.Reagents for Bile Solubility TestĢ% sodium deoxycholate (bile salt) solution Bile salts activate the autolytic enzyme which induces clearing of the culture. Bile salts lower the surface tension between the bacterial cell membrane and the medium, thus accelerating the organism’s natural autolytic process. Lysis depends on the presence of an intracellular autolytic enzyme, amidase. pneumoniae has an autolytic enzyme which can be demonstrated by allowing a broth culture to age in the incubator at 24 hours the broth is turbid after a few days the medium will become clear.īile or a solution of a bile salt (e.g., sodium desoxycholate) rapidly lyses pneumococcal colonies. Streptococcus pneumoniae is bile soluble whereas all other alpha-hemolytic streptococci are bile resistant. Bile Solubility Test is the test which differentiate Streptococcus pneumoniae (positive- soluble) from alpha-hemolytic streptococci (negative- insoluble).
0 kommentar(er)
